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| WEST-ZOL plus Western Blot Detection System |
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Description
WEST-ZOLŪ (plus) Western Blot Detection System of iNtRON is a light emitting non-radioactive method for detection of immobilized specific antigens thorough horseradish peroxidase-labeled antibodies. The HRP (horseradish peroxidase) bound to an antibody induce chemilluminescence which is detected on X-ray film.
Principles of WEST-ZOLŪ (plus) is that hydroxide ion (OH-) generated by horseradish peroxidase in aprotic media result in transition of luminol to 3-aminophthalate, which induce emission of 425-510nm light. The X-ray film exposed to the light show a specific protein band. The WEST-ZOLŪ (plus) has greater sensitivity and longer signal duration than WEST-ZOLŪ.
Characteristics
Simple step : WEST-ZOLŪ (plus) has very simple step, which consist of reaction detection solution and membrane blot and exposure to X-ray film.
Rapid reaction time : Specific protein detection may be achieved in less than 1-10 minute.
High sensitivity : WEST-ZOLŪ (plus) is able to detecting less than 1-2 pg of antigen on membrane blot, at least 8-15x more sensitive than WEST-ZOLŪ.
High resolution : WEST-ZOLŪ (plus) generate high contrast signal.
Long duration time : Signal duration time of WEST-ZOLŪ (plus) is 7-15 times longer than that of WEST-ZOLŪ.
Kit Contents
* Substrate Solution 100㎖
* Enhancer Solution 100㎖
Storage : 4C
Remarks
Dilute WEST-ZOLŪ (plus) with distilled water if the sensitivity is too high.
Cat. # 16021
WEST-ZOL
WEST-ZOLŪ (plus) Western Blot Detection System of iNtRON is a light emitting non-radioactive method for detection of immobilized specific antigens thorough horseradish peroxidase-labeled antibodies. The HRP (horseradish peroxidase) bound to an antibody induce chemilluminescence which is detected on X-ray film.
Created on 01/05/2005 01:06 AM by admin
Updated on 08/28/2005 11:29 PM by admin
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I PFU
250U
without DNTp's
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